27. Sakanaka M, Sugiyama Y, Nara M, Kitakata A, Kurihara S (Corresponding author). Functional analysis of arginine decarboxylase gene speA of Bacteroides dorei by markerless gene deletion. FEMS Microbiol Lett. in press.→Pubmed

26. Sugiyama Y, Nara M, Sakanaka M, Gotoh A, Kitakata A, Okuda S, Kurihara S (Corresponding author). (2017). Comprehensive analysis of polyamine transport and biosynthesis in the dominant human gut bacteria: potential presence of novel polyamine metabolism and transport genes. Int. J. Biochem. Cell Biol. 93:52-61. →PubMed

25. Hong-qi Xia, Yuki Kitazumi, Osamu Shirai, Hirokazu Ohta, Shin Kurihara, Kenji Kano (Corresponding author). (2017). Putrescine oxidase/peroxidase-co-immobilized and mediator-less mesoporous microelectrode for diffusion-controlled steady-state amperometric detection of putrescine. J. Electroanal. Chem. 804:128-132. →ジャーナルへのリンク

24. Gotoh A, Nara M, Sugiyama Y, Sakanaka M, Yachi H, Kitakata A, Nakagawa A, Minami H, Okuda S, Katoh T, Katayama T, Kurihara S (Corresponding author). (2017). Use of Gifu Anaerobic Medium for culturing 32 dominant species of human gut microbes and its evaluation based on short-chain fatty acids fermentation profiles. Biosci. Biotechnol. Biochem. 81:2009-2017. →PubMed

23. Yamada C (equally contributed), Gotoh A (equally contributed), Sakanaka M, Hattie M, Stubbs KA, Katayama-Ikegami A, Hirose J, Kurihara S, Arakawa T, Kitaoka M, Okuda S, Katayama T (Co-corresponding author), Fushinobu S (Co-corresponding author). (2017). Cell Chem. Biol. 24:515-524. →PubMed

22. Matsumura E, Nakagawa A, Tomabechi Y, Koyanagi T, Kumagai H, Yamamoto K, Katayama T, Sato F, and Minami H(Corresponding author). (2017). Laboratory-scale production of (S)-reticuline, an important intermediate of benzylisoquinoline alkaloids, using a bacterial-based method. Biosci. Biotechnol. Biochem. 81:396-402. →PubMed

21. Sugiyama Y, Katoh T (Corresponding author), Honda Y, Gotoh A, Ashida H, Kurihara S, Yamamoto K, Katayama T. (2017). Application study of 1,2-α-L-fucosynthase: Introduction of Fucα1,2Gal disaccharide structures on N-glycan, ganglioside, and xyloglucan oligosaccharide. Biosci. Biotechnol. Biochem. 81:283-291. →PubMed

20. Sugiyama Y, Nakamura A, Matsumoto M, Kanbe A, Sakanaka M, Higashi K, Igarashi K, Katayama T, Suzuki H, Kurihara S (Corresponding author). (2016). A novel putrescine exporter SapBCDF of Escherichia coli. J. Biol. Chem. 291:26343-26351. →PubMed

19. Katoh T, Katayama T, Tomabechi Y, Nishikawa Y, Kumada J, Matsuzaki Y, Yamamoto K. (2016). Generation of a Mutant Mucor hiemalis Endoglycosidase that Acts on Core-fucosylated N-Glycans. J. Biol. Chem. 291:23305-23317. →PubMed

18. Katayama-Ikegami A, Katayama T, Takai M, and Sakamoto T. (2016). Reference gene validation for gene expression studies using quantitative RT-PCR during berry development of ‘Aki Queen’ grapes. VITIS: Journal of Grapevine Research. 55:157-160

17. Sugiyama Y, Gotoh A, Katoh T, Honda Y, Yoshida E, Kurihara S, Ashida H, Kumagai H, Yamamoto K, Kitaoka M, Katayama T (Corresponding author). (2016). Introduction of H-antigens into oligosaccharides and sugar chains of glycoproteins using highly efficient 1,2-α-L-fucosynthase. Glycobiology. in press. →PubMed

16. Li B, Lowe-Power T, Kurihara S, Gonzales S, Naidoo J, MacMillan JB, Allen C, Michael AJ. (2016). Functional Identification of Putrescine C- and N-Hydroxylases. ACS Chemical Biology. in press. →PubMed

15. Katayama-Ikegami A, Sakamoto T, Shibuya K, Katayama T, and Gao-Takai M. (2016). Effects of abscisic acid treatment on berry coloration and expression of flavonoid biosynthesis genes in grape. American Journal of Plant Sciences. 55:157-160.

14. Sakanaka M, Sugiyama Y, Kitakata A, Katayama T, Kurihara S (Corresponding author). (2016). Carboxyspermidine decarboxylase of the prominent intestinal microbiota species Bacteroides thetaiotaomicron is required for spermidine biosynthesis and contributes to normal growth. Amino Acids. 48:2443-51. →PubMed

13. Barla F, Koyanagi T, Tokuda N, Matsui H, Katayama T, Kumagai H, Michihata T, Sasaki T, Tsuji A, and Enomoto T. (2016). The γ-aminobutyric acid-producing ability under low pH conditions of lactic acid bacteria isolated from traditional fermented foods of Ishikawa Prefecture, Japan, with a strong ability to produce ACE-inhibitory peptides. Biotechnology Reports. 10:105-110. →ジャーナルへのリンク

12. Nakagawa A, Matsumura E, Koyanagi T, Katayama T, Kawano N, Yoshimatsu K, Yamamoto K, Kumagai H, Sato F, Minami H. (2016). Total biosynthesis of opiates by stepwise fermentation using engineered Escherichia coli. Nature Communications. 7:10390. →PubMed

11. Hattie M, Ito T, Debowski AW, Arakawa T, Katayama T, Yamamoto K, Fushinobu S, Stubbs KA. (2015). Gaining insight into the catalysis by GH20 lacto-N-biosidase using small molecule inhibitors and structural analysis. Chemical Communication (Camb). 51(81):15008-11. →PumMed

10. Gotoh A, Katoh T, Sugiyama Y, Kurihara S, Honda Y, Sakurama H, Kambe T, Ashida H, Kitaoka M, Yamamoto K, Katayama T (corresponding author). (2015). Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1. Carbohydrate Research. 408:18-24. →PubMed

9. Shimada Y, Watanabe Y, Wakinaka T, Funeno Y, Kubota M, Chaiwangsri T, Kurihara S, Yamamoto K, Katayama T, Ashida H. (2015). α-N-Acetylglucosaminidase from Bifidobacterium bifidum specifically hydrolyzes α-linked N-acetylglucosamine at nonreducing terminus of O-glycan on gastric mucin. Applied Microbiology and Biotechnology. 99(9):3941-8. →PubMed

8. Viborg AH, Fredslund F, Katayama T, Nielsen SK, Svensson B, Kitaoka M, Lo Leggio L, Abou Hachem M. (2014). A β1-6/β1-3 galactosidase from Bifidobacterium animalis subsp. lactis Bl-04 gives insight into sub-specificities of β-galactoside catabolism within Bifidobacterium. Molecular Microbiology. 94(5):1024-40. →PubMed

7. Nakagawa A, Matsuzaki C, Matsumura E, Koyanagi T, Katayama T, Yamamoto K, Sato F, Kumagai H, Minami H. (2014). (R,S)-Tetrahydropapaveroline production by stepwise fermentation using engineered Escherichia coli. Scientific Reports. 4:6695. →PubMed →ジャーナルへのリンク

6. Nishihachijo M, Hirai Y, Kawano S, Nishiyama A, Minami H, Katayama T, Yasohara Y, Sato F, and Kumagai H. (2014). Asymmetric synthesis of tetrahydroisoquinolines by enzymatic Pictet-Spengler reaction. Bioscience, Biotechnology, and Biochemistry. 78(4):701-707. →PubMed →ジャーナルへのリンク

5. Kibe R*, Kurihara S*, Sakai Y, Suzuki H, Ooga T, Sawaki E, Muramatsu K, Nakamura A, Yamashita A, Kitada Y, Kakeyama M, Benno Y, Matsumoto M. (2014) Upregulation of colonic luminal polyamines produced by intestinal microbiota delays senescence in mice. Scientific Reports. 4:4548.
*These authors equally contributed to this work.

4. Takei S, Omoto C, Kitagawa K, Morishita N, Katayama T, Shigemura K, Fujisawa M, Kawabata M, Hotta H, Shirakawa T. (2014). Oral administration of genetically modified Bifidobacterium displaying HCV-NS3 multi-epitope fusion protein could induce an HCV-NS3-specific systemic immune response in mice. Vaccine. 32(25):3066-3074. →PubMed →プレスリリース

3. Matsuzaki C, Kamishima K, Matsumoto K, Koga H, Katayama T, Yamamoto K, Hisa K. (2014). Immunomodulating activity of exopolysaccharide-producing Leuconostoc mesenteroides strain NTM048 from green peas. Journal of Applied Microbiology. 116(4):980-989. →PubMed

2. Viborg AH, Katayama T, Hachem MA, Andersen MC, Nishimoto M, Clausen MH, Urashima T, Svensson B, Kitaoka M. (2014). Distinct substrate specificities of three glycoside hydrolase family 42 β-galactosidases from Bifidobacterium longum subsp. infantis ATCC 15697. Glycobiology. 24(2):208-216. →PubMed

1. Sakurama H, Kiyohara M, Wada J, Honda Y, Yamaguchi M, Fukiya S, Yokota A, Ashida H, Kumagai H, Kitaoka M, Yamamoto K, and Katayama T (corresponding author). (2013). Lacto-N-biosidase encoded by a novel gene of Bifidobacterium longum subspecies longum shows unique substrate specificity and requires a designated chaperon for its active expression. Journal of Biological Chemistry. 288(35):25194-25206.


10. 公益財団法人 三谷研究開発支援財団 助成金(代表者:阪中幹祥)

9. 科学研究費 若手研究 (A)(代表者:栗原新)

8. 科学研究費 挑戦的萌芽研究(代表者:栗原新 分担者:加納健司、川原圭博)

7. キヤノン財団 研究助成プログラム「理想の追求」(2016年採択)(代表者:栗原新 分担者:岡本成史、小柳喬)

6. 公益財団法人 旭硝子財団 自然科学系「研究奨励」(平成28年度採択)(代表者:栗原新)

5. ノボザイムズジャパン(株)2016年度研究ファンドプロジェクト(代表者:栗原新)
「Identification of novel genes encoding enzymes responsible for polyamine biosynthesis of
 Bacteroides thetaiotaomicron, the dominant intestinal bacterium of human.」

4. 公益財団法人 三谷研究開発支援財団 助成金(代表者:栗原新)

3. 科学研究費補助金 基盤研究(B)(代表者:片山高嶺 分担者:栗原新)

2. 科学研究費 挑戦的萌芽研究(代表者:栗原新 分担者:片山高嶺)
「腸内細菌学の明日を切り開く新規シンビオジェニック因子の探索 」
(2014年4月〜2016年3月) →リンク

1. 公益財団法人発酵研究所 寄附講座助成 「腸内細菌共生機構学寄附講座」



4. 栗原新. ポリアミンが仲立ちをする細菌間コミュニケーション. 化学と生物. 53:756-762. (2015). →ジャーナルへのリンク

3. 鈴木秀之, 栗原新. 大腸菌のポリアミン代謝. ポリアミン. 2:47-54 (2014). →ジャーナルへのリンク

2. 栗原新, 植村武史. 第86回日本生化学会大会 ポリアミンシンポジウム報告. ポリアミン. 1:7-10. (2014).

1. 栗原新. 大腸腸管内腔におけるポリアミンとヒト健康との関連. アンチ・エイジング医学. 9:710-716 (2013).


3. Fukiya S, Sakanaka M, Yokota A. Genetic manipulation and gene modification technologies in bifidobacteria, pp243-259. In “The bifidobacteria and related organisms: biology, taxonomy, applications” (Eds Mattarelli P, Biavati B, Holzapfel WH, and Wood JB), Academic Press, London, UK (2017). →書籍へのリンク

2. Hideyuki Suzuki* and Shin Kurihara. Polyamine catabolism in prokaryotes, pp47-60. In “Polyamines, a universal molecular nexus for growth, survival and specialised metabolism” (Eds Tomonobu Kusano and Hideyuki Suzuki). Springer, Berlin, Germany (2015).

1. Shin Kurihara* and Hideyuki Suzuki. Recent advances in bacterial polyamine transport systems, pp171-178. In “Polyamines, a universal molecular nexus for growth, survival and specialised metabolism” (Eds Tomonobu Kusano and Hideyuki Suzuki). Springer, Berlin, Germany(2015).


3. ○栗原新, 松本光晴, 鈴木秀之. 「腸内細菌のポリアミン代謝・輸送系同定の重要性」. 日本農芸化学会2015年度大会シンポジウム「ポリアミン研究のさらなる発展と新たな展開をめざした農芸化学と医学薬学との融合」. 岡山大学. 2015年3月29日.(栗原が本シンポジウムのコーディネーターも務めました。)

2. ○栗原新, 松本光晴, 鈴木秀之, 辨野義己. 「腸内細菌が作り出すシンビオジェニック因子ポリアミン 〜その機能と生産最適化〜」. 日本農芸化学会2014年度大会シンポジウム「食と健康のインターフェースを考える腸内細菌研究の最前線」. 明治大学. 2014年3月30日.

1. 片山高嶺.「 ヒトミルクオリゴ糖に作用するビフィズス菌の酵素 〜機能解析と応用〜」. 日本農芸化学会2014年度大会シンポジウム「糖鎖工学における酵素・レクチンの開発と産業応用」. 明治大学. 2014年3月30日.